ET7108-26

SULT2A1 Recombinant Rabbit Monoclonal Antibody [JG36-18]

cat.: ET7108-26

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JG36-18

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	34 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human SULT2A1 aa 140-285 / 285.
Positive control:	Mouse kidney lysates, human liver lysates, 293T, rat adrenal gland tissue, human liver carcinoma tissue, mouse liver tissue, Hela, human liver tissue, human small intestine tissue.
Subcellular location:	Cytoplasm.
Recommended Dilutions: WB IF-Cell IF-Tissue IHC-P FC	1:500-1:1,000 1:500-1:2,000 1:500-1:2,000 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: Q06520 Human \| P52843 Mouse \| P15709 Rat
Alternative names:	Alcohol/hydroxysteroid sulfotransferase Bile salt sulfotranasferase 2A1 Bile salt sulfotransferase Dehydroepiandrosterone sulfotransferase DHEA ST DHEA sulfotranasferase DHEA-ST DHEAS EC 2.8.2.14 Hst hSTa Hydroxysteroid sulfotransferase ST2 ST2A1 ST2A1_HUMAN ST2A3 STD sulfotranasferase, dehydroepiandrosterone-preferring Sulfotransferase 2A1 Sulfotransferase family 2A, dehydroepiandrosterone-preferring, member 1 Sulfotransferase family cytosolic 2A dehydroepiandrosterone (DHEA) preferring member 1 Sult2a1

Images

Fig1: Western blot analysis of SULT2A1 on mouse kidney lysates with Rabbit anti-SULT2A1 antibody (ET7108-26) at 1/1000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-26) at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of SULT2A1 on human liver lysates with Rabbit anti-SULT2A1 antibody (ET7108-26) at 1/1000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-26) at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunocytochemistry analysis of 293T cells labeling SULT2A1 with Rabbit anti-SULT2A1 antibody (ET7108-26) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SULT2A1 antibody (ET7108-26) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
	Fig4: Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue with Rabbit anti-SULT2A1 antibody (ET7108-26) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7108-26) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-SULT2A1 antibody (ET7108-26) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7108-26) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SULT2A1 antibody (ET7108-26) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7108-26) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SULT2A1 antibody (ET7108-26) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7108-26) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-SULT2A1 antibody (ET7108-26) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4)) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7108-26) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig9: Flow cytometric analysis of SULT2A1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-26, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.