UQCRFS1 Recombinant Rabbit Monoclonal Antibody [JG62-31]
cat.: ET7108-28
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JG62-31
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 21 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human UQCRFS1 aa 180-274 / 274.
Positive control: A549 cell lysate, 293 cell lysate, mouse kidney tissue lysate, 293T, LOVO, SiHa, rat skeletal muscle tissue, human colon carcinoma tissue, mouse cerebellum tissue, human appendix tissue, human kidney tissue, A549.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
  FC
1:500-1:2,000
1:500-1:2,000
1:50-1:200
1:50-1:100
1:10-1:50
Uniprot #: SwissProt: P47985 Human | Q9CR68 Mouse | P20788 Rat
Alternative names: Complex III subunit 5 Complex III subunit IX Cytochrome b c1 complex subunit Rieske mitochondrial Cytochrome b-c1 complex subunit 11 Cytochrome b-c1 complex subunit 5 Cytochrome b6f complex iron sulfur subunit, chloroplastic petC PGR1 Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein Proton gradient regulation protein 1 Rieske iron sulfur protein Rieske iron-sulfur protein RIP1 RIS1 RISP Ubiquinol cytochrome C reductase rieske iron sulphur Ubiquinol cytochrome c reductase Rieske iron-sulfur polypeptide 1 Ubiquinol-cytochrome c reductase 8 kDa protein Ubiquinol-cytochrome c reductase iron-sulfur subunit UCRI_HUMAN UQCR5 UQCRFS1
Images
ET7108-28_1.jpg Fig1: Western blot analysis of UQCRFS1 on different lysates with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/500 dilution.

Lane 1: A549 cell lysate, 10 µg/Lane
Lane 2: 293 cell lysate, 10 µg/Lane
Lane 3: Mouse kidney tissue lysate, 20 µg/Lane

Predicted band size: 21 kDa
Observed band size: 25 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-28) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7108-28_2.jpg Fig2: Immunocytochemistry analysis of 293T cells labeling UQCRFS1 with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI.
ET7108-28_3.jpg Fig3: Immunocytochemistry analysis of LOVO cells labeling UQCRFS1 with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI.
ET7108-28_4.jpg Fig4: Immunocytochemistry analysis of SiHa cells labeling UQCRFS1 with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7108-28_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-28) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-28_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-28) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-28_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-28) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-28_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-28) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-28_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-UQCRFS1 antibody (ET7108-28) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-28) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-28_10.jpg Fig10: Flow cytometric analysis of UQCRFS1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-28, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
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