IRAK2 Recombinant Rabbit Monoclonal Antibody [JG36-23]
cat.: ET7108-29
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JG36-23
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 69 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human IRAK2 aa 30-270 / 625.
Positive control: PC-3M cell lysate, A549 cell lysate, HepG2 cell lysate, PC-3M, human tonsile tissue, human prostate carcinoma tissue, human heart tissue, Jurkat.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
1:10-1:50
Uniprot #: SwissProt: O43187 Human
Alternative names: il1rak2 Interleukin 1 receptor associated kinase 2 Interleukin 1 receptor associated kinase like 2 Interleukin-1 receptor-associated kinase-like 2 IRAK 2 IRAK-2 Irak2 IRAK2_HUMAN MGC150550
Images
ET7108-29_1.jpg Fig1: Western blot analysis of IRAK2 on different lysates with Rabbit anti-IRAK2 antibody (ET7108-29) at 1/500 dilution.

Lane 1: PC-3M cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 69 kDa
Observed band size: 69 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-29) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7108-29_2.jpg Fig2: Immunocytochemistry analysis of PC-3M cells labeling IRAK2 with Rabbit anti-IRAK2 antibody (ET7108-29) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IRAK2 antibody (ET7108-29) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7108-29_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsile tissue with Rabbit anti-IRAK2 antibody (ET7108-29) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-29) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-29_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-IRAK2 antibody (ET7108-29) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-29) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-29_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-IRAK2 antibody (ET7108-29) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-29) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-29_6.jpg Fig6: Flow cytometric analysis of IRAK2 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-29, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.