WSTF Recombinant Rabbit Monoclonal Antibody [JG36-32]
cat.: ET7108-32
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JG36-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 171 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human WSTF aa 2-96 / 1,483. |
| Positive control: | SiHa lysates, K562. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UIG0 Human |
| Alternative names: | baz1b BAZ1B_HUMAN Bromodomain adjacent to zinc finger domain protein 1B hWALP 2 hWALP-2 hWALP2 transcription factor WSTF Tyrosine-protein kinase BAZ1B WALP-2 WALP2 WBRS 9 WBRS-9 WBRS9 WBSC 10 WBSC-10 WBSC10 WBSCR10 WBSCR9 Williams Beuren syndrome chromosome region 9 protein Williams syndrome transcription factor Williams-Beuren syndrome chromosomal region 10 protein Williams-Beuren syndrome chromosomal region 9 protein WSTF |
Images
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Fig1:
Western blot analysis of WSTF on SiHa lysates with Rabbit anti-WSTF antibody (ET7108-32) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 171 kDa Observed band size: 171 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-32) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of K562 cells with WSTF antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.