WSTF Recombinant Rabbit Monoclonal Antibody [JG36-32]
cat.: ET7108-32
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JG36-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 171 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human WSTF aa 2-96 / 1,483. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, L-929 cell lysate, PC-12 cell lysate, HeLa, NIH/3T3. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:100-1:250 |
| Uniprot #: | SwissProt: Q9UIG0 Human | Q9Z277 Mouse Entrez Gene: 368002 Rat |
| Alternative names: | baz1b BAZ1B_HUMAN Bromodomain adjacent to zinc finger domain protein 1B hWALP 2 hWALP-2 hWALP2 transcription factor WSTF Tyrosine-protein kinase BAZ1B WALP-2 WALP2 WBRS 9 WBRS-9 WBRS9 WBSC 10 WBSC-10 WBSC10 WBSCR10 WBSCR9 Williams Beuren syndrome chromosome region 9 protein Williams syndrome transcription factor Williams-Beuren syndrome chromosomal region 10 protein Williams-Beuren syndrome chromosomal region 9 protein WSTF |
Images
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Fig1:
Western blot analysis of WSTF on different lysates with Rabbit anti-WSTF antibody (ET7108-32) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: K-562 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: L-929 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 171 kDa Observed band size: 171 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-32) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling WSTF with Rabbit anti-WSTF antibody (ET7108-32) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-WSTF antibody (ET7108-32) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling WSTF with Rabbit anti-WSTF antibody (ET7108-32) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-WSTF antibody (ET7108-32) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.