GEF H1 Recombinant Rabbit Monoclonal Antibody [JG36-46]
cat.: ET7108-34
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG36-46 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 112 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GEF H1 aa 937-986 / 986. |
| Positive control: | HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, SH-SY5Y, HUVEC, human tonsil, mouse testis, rat brain tissue. |
| Subcellular location: | Ruffle membrane, cytoskeleton, spindle, Golgi apparatus, Cytoplasm, tight junction, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q92974 Human | Q60875 Mouse | Q5FVC2 Rat |
| Alternative names: | AA408978 ARHG2 ARHG2_HUMAN ARHGEF 2 ARHGEF-2 ARHGEF2 GEF GEF H1 GEF-H1 GEFH1 Guanine nucleotide exchange factor H1 KIAA0651 Lbcl1 Lfc LFP40 MGC95068 Microtubule-regulated Rho-GEF mKIAA0651 P40 Proliferating cell nucleolar antigen p40 Protein GEF-H1 Rho guanine nucleotide exchange factor 2 rho/rac guanine nucleotide exchange factor (GEF) 2 rho/rac guanine nucleotide exchange factor 2 rho/rac guanine nucleotide exchange factor |
Images
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Fig1:
Western blot analysis of GEF H1 on different lysates with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7108-34, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 112 kDa Observed band size: 112 kDa |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling GEF H1 with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of HUVEC cells labeling GEF H1 with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-34) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse testis with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-34) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-34) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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