GEF H1 Recombinant Rabbit Monoclonal Antibody [JG36-46]
cat.: ET7108-34
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG36-46 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 112 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GEF H1 aa 937-986 / 986. |
| Positive control: | 293T cell lysate, A431 cell lysate, SH-SY5Y, HUVEC, human tonsil, mouse testis, rat brain tissue. |
| Subcellular location: | Ruffle membrane, cytoskeleton, spindle, Golgi apparatus, Cytoplasm, tight junction, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q92974 Human | Q60875 Mouse | Q5FVC2 Rat |
| Alternative names: | AA408978 ARHG2 ARHG2_HUMAN ARHGEF 2 ARHGEF-2 ARHGEF2 GEF GEF H1 GEF-H1 GEFH1 Guanine nucleotide exchange factor H1 KIAA0651 Lbcl1 Lfc LFP40 MGC95068 Microtubule-regulated Rho-GEF mKIAA0651 P40 Proliferating cell nucleolar antigen p40 Protein GEF-H1 Rho guanine nucleotide exchange factor 2 rho/rac guanine nucleotide exchange factor (GEF) 2 rho/rac guanine nucleotide exchange factor 2 rho/rac guanine nucleotide exchange factor |
Images
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Fig1:
Western blot analysis of GEF H1 on different lysates with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/500 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 1122 kDa Observed band size: 112 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-34) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling GEF H1 with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of HUVEC cells labeling GEF H1 with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-34) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse testis with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-34) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GEF H1 antibody (ET7108-34) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-34) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.