p53 DINP1 Recombinant Rabbit Monoclonal Antibody [JG37-64]
cat.: ET7108-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JG37-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human p53 DINP1 aa 80-188 / 240. |
| Positive control: | SH-SY5Y cell lysate, HepG2 cell lysate, mouse stomach tissue lysate, human stomach tissue lysate, HepG2, HUVEC, human liver carcinoma tissue, human kidney tissue, human pancreas tissue. |
| Subcellular location: | Cytosol. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:500-1:1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q96A56 Human | Q9QXE4 Mouse | Q80YE2 Rat |
| Alternative names: | DKFZp434M1317 FLJ22139 p53 damage inducible nuclear protein 1 p53 dependent damage inducible nuclear protein 1 p53 inducible nuclear protein 1 p53 inducible p53DINP1 p53-dependent damage-inducible nuclear protein 1 p53DINP1 SIP Stress induced protein Stress-induced protein T53I1_HUMAN Teap TP53 DINP1 TP53 INP1 TP53DINP1 TP53INP1 TP53INP1A TP53INP1B Tumor protein p53 inducible nuclear protein 1 Tumor protein p53-inducible nuclear protein 1 |
Images
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Fig1:
Western blot analysis of p53 DINP1 on different lysates with Rabbit anti-p53 DINP1 antibody (ET7108-35) at 1/500 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate Lane 3: Mouse stomach tissue lysate(20 µg/Lane) Lane 4: Human stomach tissue lysate(20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-35) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HepG2 cells labeling p53 DINP1 with Rabbit anti-p53 DINP1 antibody (ET7108-35) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p53 DINP1 antibody (ET7108-35) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.