SUV39H2 Recombinant Rabbit Monoclonal Antibody [JG36-55]
cat.: ET7108-37
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JG36-55 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SUV39H2 aa 361-410 / 410. |
| Positive control: | U-2 OS cell lysate, A549 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human testis tissue, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:2,000 1:200-1:1,000 1:10-1:50 |
| Uniprot #: | SwissProt: Q9H5I1 Human | Q9EQQ0 Mouse Entrez Gene: 364785 Rat |
| Alternative names: | FLJ23414 H3 K9 HMTase 2 H3-K9-HMTase 2 Histone H3 K9 methyltransferase 2 Histone H3-K9 methyltransferase 2 Histone lysine N methyltransferase H3 lysine 9 specific 2 Histone lysine N methyltransferase SUV39H2 Histone-lysine N-methyltransferase SUV39H2 KMT1B Lysine N methyltransferase 1B Lysine N-methyltransferase 1B sSuppressor of variegation 3 9 homolog 2 (Drosophila) Su(var)3 9 Drosophila homolog of 2 Su(var)3 9 homolog 2 Su(var)3-9 homolog 2 Suppressor of variegation 3 9 homolog 2 Suppressor of variegation 3-9 homolog 2 Suv39h2 SUV92_HUMAN |
Images
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Fig1:
Western blot analysis of SUV39H2 on different lysates with Rabbit anti-SUV39H2 antibody (ET7108-37) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: A549 cell lysate Lane 3: Jurkat cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of SUV39H2 on different lysates with Rabbit anti-SUV39H2 antibody (ET7108-37) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SUV39H2 antibody (ET7108-37) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-37) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SUV39H2 antibody (ET7108-37) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SUV39H2 antibody (ET7108-37) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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