Nicastrin Recombinant Rabbit Monoclonal Antibody [JG68-33]
cat.: ET7108-38
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JG68-33 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 78 kDa |
| Isotype: | IgG |
| Immunogen: | Recomninant protein within Human Nicastrin aa 5-112 / 709. |
| Positive control: | THP-1 cell lysates, NIH/3T3 cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate. |
| Subcellular location: | Membrane, Cytoplasmic vesicle membrane, Melanosome. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q92542 Human | P57716 Mouse |
| Alternative names: | Anterior pharynx defective 2 APH 2 APH2 ATAG1874 KIAA0253 Ncstn NCT NICA_HUMAN Nicastrin RP11 517F10.1 RP11517F101 |
Images
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Fig1:
Western blot analysis of Nicastrin on THP-1 cell lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 78 kDa Observed band size: 78-150 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Nicastrin on different lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: Neuro-2a cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 78 kDa Observed band size: 78-150 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of Nicastrin on different lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Nicastrin KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 78 kDa Observed band size: 78-150 kDa Exposure time: 60 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.