SOCS2 Recombinant Rabbit Monoclonal Antibody [JG36-86]
cat.: ET7108-40
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG36-86 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SOCS2 aa 1-198 / 198. |
| Positive control: | SH-SY-5Y cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, rat epididymis tissue, LOVO, PC-3M, SH-SY-5Y. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Cell IP IHC-P |
1:500-1:2,000 1:50-1:200 1:10-1:50 1:1,000 |
| Uniprot #: | SwissProt: O14508 Human | O35717 Mouse | O88582 Rat |
| Alternative names: | CIS 2 CIS-2 CIS2 Cish 2 Cish2 Cytokine inducible SH2 protein 2 Cytokine-inducible SH2 protein 2 SOCS 2 SOCS-2 Socs2 SOCS2_HUMAN SSI 2 SSI-2 SSI2 STAT induced STAT inhibitor 2 STAT-induced STAT inhibitor 2 STATI 2 STATI2 Suppressor of cytokine signaling 2 |
Images
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Fig1:
Western blot analysis of SOCS2 on different lysates with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/1,000 dilution. Lane 1: SH-SY-5Y cell lysate Lane 2: NIH/3T3 cell lysate Lane 2: PC-12 cell lysate Lane 3: Mouse thymus tissue lysate Lane 4: Rat thymus tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 3 minutes 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-40) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-40) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of LOVO cells labeling SOCS2 with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of PC-3M cells labeling SOCS2 with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunocytochemistry analysis of SH-SY-5Y cells labeling SOCS2 with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.