WNK1 Recombinant Rabbit Monoclonal Antibody [JG36-91]
cat.: ET7108-41
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG36-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 225/250/280/300 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human WNK1 aa 33-82 / 2382. |
| Positive control: | A549 cell lysates, human skin tissue, human breast cancer tissue, mouse testis tissue, rat kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9H4A3 Human | P83741 Mouse | Q9JIH7 Rat |
| Alternative names: | Erythrocyte 65 kDa protein HSAN2 HSN2 hWNK1 KDP KIAA0344 Kinase deficient protein MGC163339 MGC163341 p65 PPP1R167 PRKWNK1 Prostate derived sterile 20 like kinase Protein kinase lysine deficient 1 Protein kinase lysine-deficient 1 Protein kinase with no lysine 1 Protein phosphatase 1, regulatory subunit 167 PSK Serine/threonine protein kinase WNK1 1 Serine/threonine protein kinase WNK1 2 Serine/threonine protein kinase WNK1 Serine/threonine-protein kinase WNK1 With no K WNK lysine deficient protein kinase 1 WNK lysine deficient protein kinase 1 isoform WNK1 WNK1_HUMAN |
Images
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Fig1:
Western blot analysis of WNK1 on A549 cell lysate lysates with Rabbit anti-WNK1 antibody (ET7108-41) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 225/250/280/300 kDa kDa Observed band size: 250/300 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-41) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-WNK1 antibody (ET7108-41) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-41) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-WNK1 antibody (ET7108-41) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-41) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-WNK1 antibody (ET7108-41) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-41) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-WNK1 antibody (ET7108-41) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-41) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.