Tetranectin Recombinant Rabbit Monoclonal Antibody [JG37-16]
cat.: ET7108-45
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
Clonality: Monoclonal
Clone number: JG37-16
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Tetranectin aa 110-202 / 202.
Positive control: Rat skin tissue lysate, human skin tissue lysate, HepG2, MCF-7, human liver carcinoma tissue, human kidney tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IP

1:1,000-1:5,000
1:50-1:200
1:50-1:200
1:10-1:50
Uniprot #: SwissProt: P05452 Human | P43025 Mouse
Alternative names: C type lectin domain family 3 member B C-type lectin domain family 3 member B Clec3b Plasminogen kringle 4 binding protein Plasminogen kringle 4-binding protein TETN_HUMAN Tetranectin (plasminogen binding protein) Tetranectin TN TNA
Images
ET7108-45_1.jpg Fig1: Western blot analysis of Tetranectin on different lysates with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/500 dilution.

Lane 1: Rat skin tissue lysate
Lane 2: Human skin tissue lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-45) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7108-45_2.jpg Fig2: Immunocytochemistry analysis of HepG2 cells labeling Tetranectin with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7108-45_3.jpg Fig3: Immunocytochemistry analysis of MCF-7 cells labeling Tetranectin with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7108-45_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-45) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-45_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-45) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.