Fibrinogen gamma chain Recombinant Rabbit Monoclonal Antibody [JG37-18]
cat.: ET7108-46
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG37-18 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Fibrinogen gamma chain aa 361-453 / 453. |
| Positive control: | Human plasma lysates, human colon tissue, HepG2. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P02679 Human |
| Alternative names: | FGG FIBG_HUMAN Fibrinogen gamma chain Fibrinogen gamma polypeptide fibrinogen gamma-b chain |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Fibrinogen gamma chain antibody (ET7108-46) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-46) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Flow cytometric analysis of Fibrinogen gamma chain was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-46, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow). |
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Fig3:
Western blot analysis of Fibrinogen gamma chain on different lysates with Rabbit anti-Fibrinogen gamma chain antibody (ET7108-46) at 1/2,000 dilution. Lane 1: Human plasma lysate Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7108-46, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti- Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 55 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.