Cullin 2 Recombinant Rabbit Monoclonal Antibody [JG72-31]
cat.: ET7108-47
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JG72-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 89 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal Human Cullin 2 . |
| Positive control: | PC-3M cell lysate, k562 tissue lysate, rat testis tissue, human tonsil tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:2,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: Q13617 Human | Q9D4H8 Mouse Entrez Gene: 361258 Rat |
| Alternative names: | Cul2 CUL 2 CUL-2 CUL2 CUL2_HUMAN cullin 2 cullin homolog 2 Cullin-2 MGC131970 |
Images
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Fig1:
Western blot analysis of Cullin 2 on different lysates with Rabbit anti-Cullin 2 antibody (ET7108-47) at 1/500 dilution. Lane 1: PC-3M cell lysate Lane 2: K562 tissue lysate (20 µg/Lane) Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 92 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-47) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Cullin 2 antibody (ET7108-47) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-47) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Cullin 2 antibody (ET7108-47) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-47) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.