RanGAP1 Recombinant Rabbit Monoclonal Antibody [JG37-41]
cat.: ET7108-48
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JG37-41
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 63 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human RanGAP1 aa 1-50 / 587.
Positive control: HeLa cell lysate, A431 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human testis tissue, mouse testis tissue, rat testis tissue, MCF7, NIH/3T3, PC-12.
Subcellular location: Cytoplasm. Cytoskeleton. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
1:1,000
1:100
1:100
1:50-1:200
1:100
1:10-1:50
Uniprot #: SwissProt: P46060 Human | P46061 Mouse
Entrez Gene: 362965 Rat
Alternative names: Fug 1 Fug1 GTPase-activating protein, RAN, 1 KIAA1835 MGC20266 OTTHUMP00000028918 OTTHUMP00000198755 OTTHUMP00000198756 OTTHUMP00000198757 OTTHUMP00000198758 RAGP1_HUMAN Ran 1 RAN GTPase activating protein 1 Ran GTPase-activating protein 1 Ran1 RANGAP 1 RANGAP RanGAP1 SD Segregation distorter homolog Segregation distortion
Images
ET7108-48_1.jpg Fig1: Western blot analysis of RanGAP1 on different lysates with Rabbit anti-RanGAP1 antibody (ET7108-48) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: SH-SY5Y cell lysate
Lane 5: Jurkat cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 63 kDa
Observed band size: 63/80 kDa

Exposure time: 3 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7108-48_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-RanGAP1 antibody (ET7108-48) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-48_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-RanGAP1 antibody (ET7108-48) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-48_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-RanGAP1 antibody (ET7108-48) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-48_5.jpg Fig5: Immunocytochemistry analysis of MCF7 cells labeling RanGAP1 with Rabbit anti-RanGAP1 antibody (ET7108-48) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RanGAP1 antibody (ET7108-48) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET7108-48_6.jpg Fig6: Immunocytochemistry analysis of NIH/3T3 cells labeling RanGAP1 with Rabbit anti-RanGAP1 antibody (ET7108-48) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RanGAP1 antibody (ET7108-48) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET7108-48_7.jpg Fig7: Immunocytochemistry analysis of PC-12 cells labeling RanGAP1 with Rabbit anti-RanGAP1 antibody (ET7108-48) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RanGAP1 antibody (ET7108-48) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET7108-48_8.jpg Fig8: Flow cytometric analysis of MCF7 cells labeling RanGAP1.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7108-48, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET7108-48_9.jpg Fig9: Flow cytometric analysis of NIH/3T3 cells labeling RanGAP1.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7108-48, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET7108-48_10.jpg Fig10: Flow cytometric analysis of PC-12 cells labeling RanGAP1.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7108-48, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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