Anti-ILF3 antibody [JG37-63]
cat.: ET7108-49
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, ICC, IF, IHC-P, FC
Clonality: Monoclonal
Clone number: JG37-63
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 75/95 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal Human ILF3 .
Positive control: Rat heart tissue lysate, 293, A431, SH-SY-5Y, SiHa, human tonsil tissue, human colon tissue, human prostate cancer tissue, mouse testis tissue, mouse kidney tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC
  IP

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
1:10-1:50
Uniprot #: SwissProt: Q12906 Human | Q9Z1X4 Mouse | Q9JIL3 Rat
Alternative names: CBTF Double stranded RNA binding protein 76 Double-stranded RNA-binding protein 76 DRBF DRBP76 ILF3 ILF3_HUMAN Interleukin enhancer binding factor 3 Interleukin enhancer-binding factor 3 M phase phosphoprotein 4 M-phase phosphoprotein 4 MPHOSPH4 MPP4 NF AT 90 NF-AT-90 NF110 NF90 NFAR Nuclear factor associated with dsRNA Nuclear factor of activated T cells 90 kDa Nuclear factor of activated T-cells 90 kDa TCP80 Translational control protein 80
Images
ET7108-49_1.jpg Fig1: Western blot analysis of ILF3 on rat heart tissue (1) and 293 cell (2) lysate using anti-ILF3 antibody at 1/1,000 dilution.
ET7108-49_2.jpg Fig2: ICC staining ILF3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7108-49_3.jpg Fig3: ICC staining ILF3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7108-49_4.jpg Fig4: ICC staining ILF3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7108-49_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
ET7108-49_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
ET7108-49_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
ET7108-49_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
ET7108-49_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
ET7108-49_10.jpg Fig10: Flow cytometric analysis of K562 cells with ILF3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.