HIF1AN Recombinant Rabbit Monoclonal Antibody [JG37-66]
cat.: ET7108-50
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG37-66 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HIF1AN aa 1-50 / 349. |
| Positive control: | A549 cell lysate, Jurkat cell lysate, LOVO, SiHa, human prostate carcinoma tissue. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9NWT6 Human |
| Alternative names: | DKFZp762F1811 Factor inhibiting HIF-1 Factor inhibiting HIF1 FIH 1 FIH-1 FIH1 FLJ20615 FLJ22027 HIF1AN HIF1N_HUMAN Hypoxia inducible factor 1 alpha inhibitor Hypoxia inducible factor 1 alpha subunit inhibitor Hypoxia inducible factor asparagine hydroxylase Hypoxia-inducible factor 1-alpha inhibitor Hypoxia-inducible factor asparagine hydroxylase Peptide aspartate beta dioxygenase |
Images
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Fig1:
Western blot analysis of HIF1AN on different lysates with Rabbit anti-HIF1AN antibody (ET7108-50) at 1/500 dilution. Lane 1: A549 cell lysates Lane 2: Jurkat cell lysates Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-50) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of LOVO cells labeling HIF1AN with Rabbit anti-HIF1AN antibody (ET7108-50) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HIF1AN antibody (ET7108-50) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of SiHa cells labeling HIF1AN with Rabbit anti-HIF1AN antibody (ET7108-50) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HIF1AN antibody (ET7108-50) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-HIF1AN antibody (ET7108-50) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-50) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of LOVO cells with HIF1AN antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.