P2Y6 Recombinant Rabbit Monoclonal Antibody [JG37-77]
cat.: ET7108-52
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JG37-77 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human P2Y6 aa 279-328 / 328. |
| Positive control: | A549, human liver tissue, human kidney tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IP IHC-P IF-Cell |
1:500-1:2,000 1:10-1:50 1:200-1:1,000 1:50 |
| Uniprot #: | SwissProt: Q15077 Human |
| Alternative names: | G coupled nucleotide receptor G protein coupled 6 MGC15335 P2 purinoceptor P2ry6 P2RY6_HUMAN P2Y purinoceptor 6 P2Y6 P2Y6 receptor PP2891 Purinergic receptor P2Y6 pyrimidinergic receptor P2Y Pyrimidinergic receptor P2Y, G protein coupled, 6 |
Images
|
Fig1:
Western blot analysis of P2Y6 on A549 (Human lung adenocarcinoma cell) cell lysate with Rabbit anti-P2Y6 antibody (ET7108-52) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 62 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7108-52, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 36.4 kDa Observed band size: 40 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET7108-52, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET7108-52, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.