P2Y6 Recombinant Rabbit Monoclonal Antibody [JG37-77]
cat.: ET7108-52
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JG37-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human P2Y6 aa 279-328 / 328. |
| Positive control: | SK-Br-3, A549, human liver tissue, human colon tissue, human kidney tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IP IHC-P IF-Cell |
1:500-1:2,000 1:10-1:50 1:50-1:200 1:50 |
| Uniprot #: | SwissProt: Q15077 Human |
| Alternative names: | G coupled nucleotide receptor G protein coupled 6 MGC15335 P2 purinoceptor P2ry6 P2RY6_HUMAN P2Y purinoceptor 6 P2Y6 P2Y6 receptor PP2891 Purinergic receptor P2Y6 pyrimidinergic receptor P2Y Pyrimidinergic receptor P2Y, G protein coupled, 6 |
Images
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Fig1:
Western blot analysis of P2Y6 on different lysates with Rabbit anti-P2Y6 antibody (ET7108-52) at 1/1,000 dilution. Line 1: SK-Br-3 cell lysates Line 2: A549 cell lysates Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-52) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-P2Y6 antibody (ET7108-52) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-52) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-P2Y6 antibody (ET7108-52) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-52) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-P2Y6 antibody (ET7108-52) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-52) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.