SENP1 Recombinant Rabbit Monoclonal Antibody [JG37-79]
cat.: ET7108-53
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JG37-79
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 73 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human SENP1 aa 380-521 / 644.
Positive control: K562 cell lysates, LOVO, SH-SY-5Y, SiHa, human liver tissue, human small Intestine tissue, Daudi.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9P0U3 Human
Alternative names: SENP 1 SENP1 SENP1_HUMAN Sentrin specific protease 1 Sentrin-specific protease 1 Sentrin/SUMO specific protease 1 Sentrin/SUMO specific protease Sentrin/SUMO specific protease SENP 1 Sentrin/SUMO specific protease SENP1 Sentrin/SUMO-specific protease SENP1 SUMO1/sentrin specific peptidase 1 SUMO1/sentrin specific protease 1 SuPr 2 SuPr2
Images
ET7108-53_1.jpg Fig1: Western blot analysis of SENP1 on different lysates with Rabbit anti-SENP1 antibody (ET7108-53) at 1/2,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-SENP1 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 73 kDa
Observed band size: 73 kDa

Exposure time: 60 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-53) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7108-53_2.jpg Fig2: Western blot analysis of SENP1 on K562 cell lysates with Rabbit anti-SENP1 antibody (ET7108-53) at 1/1,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 73 kDa
Observed band size: 75 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-53) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7108-53_3.jpg Fig3: Immunocytochemistry analysis of LOVO cells labeling SENP1 with Rabbit anti-SENP1 antibody (ET7108-53) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SENP1 antibody (ET7108-53) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7108-53_4.jpg Fig4: Immunocytochemistry analysis of SH-SY-5Y cells labeling SENP1 with Rabbit anti-SENP1 antibody (ET7108-53) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SENP1 antibody (ET7108-53) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI.
ET7108-53_5.jpg Fig5: Immunocytochemistry analysis of SiHa cells labeling SENP1 with Rabbit anti-SENP1 antibody (ET7108-53) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SENP1 antibody (ET7108-53) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET7108-53_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SENP1 antibody (ET7108-53) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-53) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-53_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human small Intestine tissue with Rabbit anti-SENP1 antibody (ET7108-53) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-53) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-53_8.jpg Fig8: Flow cytometric analysis of Daudi cells with SENP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.