BAP31 Recombinant Rabbit Monoclonal Antibody [JG37-81]
cat.: ET7108-54
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JG37-81
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 28 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human BAP31 aa 81-220 / 246.
Positive control: A431 cell lysate, SK-Br-3 cell lysate, A431, A549, PC-3M, mouse testis tissue, human testis tissue, Hela.
Subcellular location: Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P51572 Human | Q61335 Mouse
Alternative names: 6C6 AG 6C6 AG tumor associated antigen 6C6-AG tumor-associated antigen 6C6AG 6C6AG tumor associated antigen Accessory protein BAP 31 Accessory protein BAP31 B cell receptor associated protein 31 B-cell receptor-associated protein 31 BA31 BAP 31 Bap31 BAP31_HUMAN BCAP 31 BCAP31 BCR associated protein Bap 31 BCR associated protein Bap31 BCR-associated protein 31 CDM CDM protein DXS1357E MS950 p28 p28 Bap31 Protein CDM RP23-329M9.5
Images
ET7108-54_1.jpg Fig1: Western blot analysis of BAP31 on different lysates with Rabbit anti-BAP31 antibody (ET7108-54) at 1/1,000 dilution.

Lane 1: A431 cell lysate
Lane 2: SK-Br-3 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 28 kDa
Observed band size: 28 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7108-54_2.jpg Fig2: ICC staining of BAP31 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-54, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-54_3.jpg Fig3: ICC staining of BAP31 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-54, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-54_4.jpg Fig4: ICC staining of BAP31 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-54, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-54_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-BAP31 antibody (ET7108-54) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-54) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-54_6.jpg Fig6: Flow cytometric analysis of BAP31 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-54, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.