Annexin IV Recombinant Rabbit Monoclonal Antibody [JG37-83]
cat.: ET7108-56
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JG37-83
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 36 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Human Annexin IV aa 10-107 / 319.
Positive control: Human liver tissue lysates, A549, HepG2, SiHa, human colon carcinoma tissue, human kidney tissue.
Subcellular location: Secreted. Nucleus. Plasma Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P09525 Human
Alternative names: 35 beta calcimedin 35-beta calcimedin AIV Annexin 4 Annexin A4 Annexin IV (placental anticoagulant protein II) Annexin IV Annexin IV placental anticoagulant protein II Annexin-4 AnnexinA4 AnnexinIV ANX 4 ANX A4 ANX4 ANXA 4 ANXA4 ANXA4 protein ANXA4_HUMAN Carbohydrate binding protein P33/P41 Carbohydrate-binding protein p33/p41 Chromobindin 4 Chromobindin-4 Chromobindin4 DKFZp686H02120 Endonexin I EndonexinI Lipocortin IV LipocortinIV MGC75105 OTTHUMP00000160052 OTTHUMP00000202207 P32.5 P33/41 PAP II PAP-II PAPII PIG 28 PIG28 Placental anticoagulant protein II PP4 X PP4-X PP4X Proliferation inducing gene 28 Proliferation inducing protein 28 Protein II Xanx-4 ZAP 36 ZAP36
Images
ET7108-56_1.jpg Fig1: Western blot analysis of Annexin IV on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7108-56_2.jpg Fig2: ICC staining of Annexin IV in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-56, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-56_3.jpg Fig3: ICC staining of Annexin IV in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-56, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-56_4.jpg Fig4: ICC staining of Annexin IV in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-56, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-56_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Annexin IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-56_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Annexin IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-56_7.jpg Fig7: Flow cytometric analysis of Annexin IV was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-56, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.