EGR2 Recombinant Rabbit Monoclonal Antibody [JG78-39]
cat.: ET7108-57
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JG78-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human EGR2 aa 2-96 / 476. |
| Positive control: | SH-SY5Y cell lysate, HepG2 cell lysate, Daudi cell lysate, RAW264.7 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: P11161 Human |
| Alternative names: | AT591 CMT1D CMT4E DKFZp686J1957 E3 SUMO-protein ligase EGR2 Early growth response 2 Early growth response protein 2 EGR-2 egr2 EGR2_HUMAN FLJ14547 KROX 20 Drosophila homolog Krox 20 homolog Drosophila KROX-20, Drosophila, homolog (early growth response-2) KROX20 Krox20 protein Zinc finger protein Krox-20 |
Images
|
Fig1:
Western blot analysis of EGR2 on different lysates with Rabbit anti-EGR2 antibody (ET7108-57) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate Lane 3: Daudi cell lysate Lane 4: RAW264.7 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-57) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.