PICK1 Recombinant Rabbit Monoclonal Antibody [JG30-80]
cat.: ET7108-60
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, FC
Clonality: Monoclonal
Clone number: JG30-80
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 47 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PICK1 aa 1-50 / 415.
Positive control: SK-Br-3 cell lysate, 293 cell lysate, 293T.
Subcellular location: cytoskeleton, perinuclear region, membrane, postsynaptic density, synaptosome.
Recommended Dilutions:
  WB
  FC
  IP

1:500-1:2,000
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q9NRD5 Human
Alternative names: dJ1039K5 MGC15204 OTTHUMP00000028509 PICK 1 Pick1 PICK1_HUMAN PRKCA binding protein PRKCA-binding protein PRKCABP Protein interacting with C kinase 1 Protein interacting with PRKCA Protein interacting with PRKCA 1 Protein kinase C alpha binding protein Protein kinase C-alpha-binding protein Protein that interacts with C kinase 1
Images
ET7108-60_1.jpg Fig1: Western blot analysis of PICK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SK-Br-3 cell lysate
Lane 2: 293 cell lysate
ET7108-60_2.jpg Fig2: Flow cytometric analysis of PICK1 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-60, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.