PICK1 Recombinant Rabbit Monoclonal Antibody [JG30-80]
cat.: ET7108-60
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JG30-80 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PICK1 aa 1-50 / 415. |
| Positive control: | SK-Br-3 cell lysate, 293 cell lysate, 293T. |
| Subcellular location: | cytoskeleton, perinuclear region, membrane, postsynaptic density, synaptosome. |
| Recommended Dilutions:
WB FC IP |
1:500-1:2,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9NRD5 Human |
| Alternative names: | dJ1039K5 MGC15204 OTTHUMP00000028509 PICK 1 Pick1 PICK1_HUMAN PRKCA binding protein PRKCA-binding protein PRKCABP Protein interacting with C kinase 1 Protein interacting with PRKCA Protein interacting with PRKCA 1 Protein kinase C alpha binding protein Protein kinase C-alpha-binding protein Protein that interacts with C kinase 1 |
Images
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Fig1:
Western blot analysis of PICK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-Br-3 cell lysate Lane 2: 293 cell lysate |
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Fig2: Flow cytometric analysis of PICK1 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-60, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.