PICK1 Recombinant Rabbit Monoclonal Antibody [JG30-80]
cat.: ET7108-60
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JG30-80 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PICK1 aa 1-50 / 415. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate,293T cell lysate, LNCap cell lysate. |
| Subcellular location: | cytoskeleton, perinuclear region, membrane, postsynaptic density, synaptosome. |
| Recommended Dilutions:
WB FC IP |
1:5,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9NRD5 Human |
| Alternative names: | dJ1039K5 MGC15204 OTTHUMP00000028509 PICK 1 Pick1 PICK1_HUMAN PRKCA binding protein PRKCA-binding protein PRKCABP Protein interacting with C kinase 1 Protein interacting with PRKCA Protein interacting with PRKCA 1 Protein kinase C alpha binding protein Protein kinase C-alpha-binding protein Protein that interacts with C kinase 1 |
Images
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Fig1:
Western blot analysis of PICK1 on different lysates with Rabbit anti-PICK1 antibody (ET7108-60) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: MCF7 (Human breast cancer cell) cell lysate Lane 3: 293T (Human embryonic kidney cell) cell lysate Lane 4: LNCaP (Human prostate cancer cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7108-60, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 47 kDa Observed band size: 47 kDa |
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Fig2: Flow cytometric analysis of PICK1 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-60, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.