OS9 Recombinant Rabbit Monoclonal Antibody [JG38-07]
cat.: ET7108-61
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: JG38-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 76 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human OS9 aa 561-667 / 667.
Positive control: Mouse testis tissue lysate, rat testis tissue lysate, rat epididymis tissue, human breast carcinoma tissue, human placenta tissue, mouse testis tissue.
Subcellular location: Endoplasmic reticulum.
Recommended Dilutions:
  WB
  IHC-P
  IP
1:1,000-1:2,000
1:50-1:200
1:10-1:50
Uniprot #: SwissProt: Q13438 Human | Q8K2C7 Mouse | Q5RKH6 Rat
Alternative names: Amplified in osteosarcoma 9 amplified in osteosarcoma ERLEC2 OS-9 Os9 OS9_HUMAN Osteosarcoma amplified 9 endoplasmic reticulum lectin Protein OS-9
Images
ET7108-61_1.jpg Fig1: Western blot analysis of OS9 on different lysates with Rabbit anti-OS9 antibody (ET7108-61) at 1/2,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-OS9 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 76 kDa
Observed band size: 76 kDa

Exposure time: 62 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-61) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7108-61_2.jpg Fig2: Western blot analysis of OS9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-61, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse testis tissue lysate
Lane 2: Rat testis tissue lysate
ET7108-61_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-OS9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-61_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-OS9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-61_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-OS9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-61_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-OS9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.