Gemin 1 Recombinant Rabbit Monoclonal Antibody [JG38-19]
cat.: ET7108-63
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JG38-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 32 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Gemin aa 83-220 / 294. |
| Positive control: | SiHa, rat testis tissue, mouse brain tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:1,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: Q16637 Human |
| Alternative names: | BCD541 Component of gems 1 Gemin 1 Gemin-1 OTTHUMP00000125198 OTTHUMP00000223567 OTTHUMP00000223568 OTTHUMP00000224066 OTTHUMP00000226924 SMA 1 SMA 2 SMA 3 SMA 4 SMA SMA@ SMA1 SMA2 SMA3 SMA4 SMN SMN_HUMAN SMN1 SMN2 SMNT Survival motor neuron protein Survival of motor neuron 1, telomeric T-BCD541 |
Images
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Fig1:
Western blot analysis of Gemin 1 on SiHa cell lysates with Rabbit anti-Gemin 1 antibody (ET7108-63) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 32 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-63) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Gemin 1 antibody (ET7108-63) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-63) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Gemin 1 antibody (ET7108-63) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-63) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.