Alcohol Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JG82-31]
cat.: ET7108-64
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JG82-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Alcohol Dehydrogenase aa 2-107 / 375. |
| Positive control: | Rat liver tissue lysates, HepG2, human liver tissue, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: P07327 Human | P00329 Mouse | P06757 Rat |
| Alternative names: | ADH alpha subunit ADH ADH1 ADH1A ADH1A_HUMAN Alcohol dehydrogenase 1 (class I), alpha polypeptide Alcohol dehydrogenase 1 Alcohol dehydrogenase 1A (class I), alpha polypeptide Alcohol dehydrogenase 1A Alcohol dehydrogenase subunit alpha Aldehyde reductase |
Images
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Fig1:
Immunocytochemistry analysis of HepG2 cells labeling Alcohol Dehydrogenase with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-64) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-64) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-64) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of Alcohol Dehydrogenase on different lysates with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/1,000 dilution. Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7108-64, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 39.9 kDa Observed band size: 40 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.