STUB1 Recombinant Rabbit Monoclonal Antibody [JG38-22]
cat.: ET7108-65
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: JG38-22
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 35 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human STUB1 aa 10-59 / 303.
Positive control: HeLa cell lysate, HEK-293 cell lysate, human brain tissue lysate, human heart tissue, mouse heart tissue, rat heart tissue, 293T, LOVO, MCF-7, SH-SY5Y.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
  IP
1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q9UNE7 Human | Q9WUD1 Mouse
Entrez Gene: 287155 Rat
Alternative names: Antigen NY CO 7 Antigen NY-CO-7 C terminus of Hsp70-interacting protein Carboxy terminus of Hsp70 interacting protein Carboxy terminus of Hsp70-interacting protein Carboxy terminus of Hsp70p interacting protein CHIP CHIP_HUMAN CLL associated antigen KW 8 CLL-associated antigen KW-8 E3 ubiquitin protein ligase CHIP E3 ubiquitin-protein ligase CHIP Heat shock protein A binding protein 2 (c terminal) HSPABP2 NY CO 7 PP1131 SDCCAG7 Serologically defined colon cancer antigen 7 STIP1 homology and U Box containing protein 1 STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase STIP1 homology and U box-containing protein 1 STUB 1 STUB1 UBOX 1 UBOX1
Images
ET7108-65_1.jpg Fig1: Western blot analysis of STUB1 on different lysates with Rabbit anti-STUB1 antibody (ET7108-65) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HEK-293 cell lysate (15 µg/Lane)
Lane 3: Human brain tissue lysate (30 µg/Lane)

Predicted band size: 35 kDa
Observed band size: 34 kDa

Exposure time: 14 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7108-65_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-STUB1 antibody (ET7108-65) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-65) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-65_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-STUB1 antibody (ET7108-65) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-65) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-65_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-STUB1 antibody (ET7108-65) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-65) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-65_5.jpg Fig5: ICC staining of STUB1 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-65, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-65_6.jpg Fig6: ICC staining of STUB1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-65, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-65_7.jpg Fig7: ICC staining of STUB1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-65, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-65_8.jpg Fig8: Flow cytometric analysis of STUB1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-65, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.