GRIM19 Recombinant Rabbit Monoclonal Antibody [JG82-33]
cat.: ET7108-66
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JG82-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GRIM19 aa 1-144 / 144. |
| Positive control: | HeLa cell lysate, Ramos cell lysate, 293T cell lysate, Jurkat cell lysate, MCF7 cell lysate, human kidney tissue, human liver tissue, human prostate cancer tissue, HepG2. |
| Subcellular location: | Mitochondrion. Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9P0J0 Human |
| Alternative names: | 2700054G14Rik AU022060 B16.6 CDA016 Cell death regulatory protein Cell death regulatory protein GRIM-19 CGI-39 CGI39 protein CI-B16.6 Complex I-B16.6 FLJ58045 FLJ59191 Gene associated with retinoic and IFN-induced mortality 19 protein Gene associated with retinoic and interferon-induced mortality 19 protein Gene associated with retinoic interferon induced mortality 19 protein GRIM-19 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 NADH ubiquinone oxidoreductase B16.6 subunit NADH-ubiquinone oxidoreductase B16.6 subunit NDUAD_HUMAN NDUFA13 RGD1565358 |
Images
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Fig1:
Western blot analysis of GRIM19 on different lysates with Rabbit anti-GRIM19 antibody (ET7108-66) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Ramos cell lysate Lane 3: 293T cell lysate Lane 4: Jurkat cell lysate Lane 5: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GRIM19 antibody (ET7108-66) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-66) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GRIM19 antibody (ET7108-66) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-66) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-GRIM19 antibody (ET7108-66) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-66) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of HepG2 cells labeling GRIM19 with Rabbit anti-GRIM19 antibody (ET7108-66) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GRIM19 antibody (ET7108-66) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.