Cpn10 Recombinant Rabbit Monoclonal Antibody [JG82-34]
cat.: ET7108-67
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: JG82-34
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 11 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Cpn10 aa 10-102 / 102.
Positive control: HeLa cell lysate, A549 cell lysate, Neuro-2a cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, rat testis tissue, human liver carcinoma tissue, human colon tissue, mouse kidney tissue.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IP
  IHC-P
1:2,000
1:10-1:50
1:50-1:200
Uniprot #: SwissProt: P61604 Human | Q64433 Mouse | P26772 Rat
Alternative names: 10 kDa chaperonin 10 kDa heat shock protein mitochondrial 10 kDa heat shock protein, mitochondrial CH10_HUMAN Chaperonin 10 Chaperonin 10 homolog CPN10 cpn10 homolog Early pregnancy factor Early-pregnancy factor EPF GROES GroES homolog Heat shock 10kD protein 1 chaperonin 10 Heat shock 10kDa protein 1 Heat shock 10kDa protein 1 chaperonin 10 Heat shock protein family E (Hsp10) member Heat-shock 10-kD protein Hsp10 Hspe1
Images
ET7108-67_1.jpg Fig1: Western blot analysis of Cpn10 on different lysates with Rabbit anti-Cpn10 antibody (ET7108-67) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: Neuro-2a cell lysate (20 µg/Lane)
Lane 4: Mouse kidney tissue lysate (40 µg/Lane)
Lane 5: Rat kidney tissue lysate (40 µg/Lane)

Predicted band size: 11 kDa
Observed band size: 11 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-67) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7108-67_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Cpn10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-67_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cpn10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-67_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Cpn10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-67_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cpn10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.