Gli3 Recombinant Rabbit Monoclonal Antibody [JG83-39]
cat.: ET7108-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JG83-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 190 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Gli3 aa 1261-1580 / 1580. |
| Positive control: | PC-3M cell lysates. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: P10071 Human |
| Alternative names: | ACLS DNA binding protein GCPS Gli 3 GLI family zinc finger 3 GLI Kruppel family member GLI 3 GLI Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome) GLI Kruppel family member GLI3 GLI3 GLI3 C-terminally truncated form GLI3 form of 190 kDa GLI3 form of 83 kDa GLI3 full length protein GLI3-190 GLI3-83 GLI3_HUMAN GLI3FL Glioma associated oncogene family zinc finger 3 Oncogene GLI3 PAP A PAPA 1 PAPA PAPA1 PAPB PHS PPD IV PPDIV Transcriptional activator GLI3 Transcriptional repressor GLI3R Zinc finger protein GLI 3 Zinc finger protein GLI3 |
Images
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Fig1: Western blot analysis of Gli3 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.