Xanthine Oxidase Recombinant Rabbit Monoclonal Antibody [JG38-40]
cat.: ET7108-69
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG38-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 146 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Xanthine Oxidase aa 182-313 / 1333. |
| Positive control: | Human liver tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Cytoplasm. Peroxisome. Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P47989 Human | Q00519 Mouse | P22985 Rat |
| Alternative names: | Xanthine dehydrogenase Xanthine dehydrogenase/oxidase Xanthine oxidase Xanthine oxidoreductase XD XDH XDH_HUMAN xdha XO xor |
Images
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Fig1:
Western blot analysis of Xanthine Oxidase on different lysates with Rabbit anti-Xanthine Oxidase antibody (ET7108-69) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 146 kDa Observed band size: 146 kDa Exposure time: Lane 1: 1 minute 2 seconds; Lane 2-3: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-69) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Xanthine Oxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Xanthine Oxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Xanthine Oxidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.