Anti-hUPF1 antibody [JG38-44]
cat.: ET7108-70
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, ICC, IF, IP, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG38-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/mL. |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 124 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal Human hUPF1 . |
| Positive control: | PC-3M, A431, LOVO, human colon tissue, human prostate cancer tissue, human kidney tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB ICC/IF IHC-P FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 1:10-1:50 |
| Uniprot #: | SwissProt: Q92900 Human | Q9EPU0 Mouse |
| Alternative names: | ATP dependent helicase RENT1 ATP-dependent helicase RENT1 Delta helicase FLJ43809 FLJ46894 HUPF 1 hUpf1 KIAA0221 Nonsense mRNA reducing factor 1 NORF 1 NORF1 pNORF 1 pNORF1 Regulator of nonsense transcripts 1 RENT 1 RENT1 RENT1_HUMAN Smg 2 Smg 2 homolog nonsense mediated mRNA decay factor UP Frameshift 1 Up frameshift mutation 1 homolog (S. cerevisiae) Up frameshift mutation 1 homolog Up frameshift suppressor 1 homolog Up-frameshift suppressor 1 homolog UPF 1 UPF 1 regulator of nonsense transcripts homolog upf1 UPF1 regulator of nonsense transcripts homolog UPF1 RNA helicase and ATPase Yeast Upf1p homolog |
Images
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Fig1: Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1,000 dilution. |
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Fig2: ICC staining hUPF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining hUPF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining hUPF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- hUPF1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-hUPF1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- hUPF1 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- hUPF1 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of PC-3M cells with hUPF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.