hUPF1 Recombinant Rabbit Monoclonal Antibody [JG38-44]
cat.: ET7108-70
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IP, IHC-P, FC
Clonality: Monoclonal
Clone number: JG38-44
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 124 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human hUPF1 aa 1-120 / 1129.
Positive control: PC-3M cell lysates, A431, LOVO, PC-3M, human colon tissue, human prostate carcinoma tissue, human kidney tissue, mouse brain tissue, Hela.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
1:10-1:50
Uniprot #: SwissProt: Q92900 Human | Q9EPU0 Mouse
Alternative names: ATP dependent helicase RENT1 ATP-dependent helicase RENT1 Delta helicase FLJ43809 FLJ46894 HUPF 1 hUpf1 KIAA0221 Nonsense mRNA reducing factor 1 NORF 1 NORF1 pNORF 1 pNORF1 Regulator of nonsense transcripts 1 RENT 1 RENT1 RENT1_HUMAN Smg 2 Smg 2 homolog nonsense mediated mRNA decay factor UP Frameshift 1 Up frameshift mutation 1 homolog (S. cerevisiae) Up frameshift mutation 1 homolog Up frameshift suppressor 1 homolog Up-frameshift suppressor 1 homolog UPF 1 UPF 1 regulator of nonsense transcripts homolog upf1 UPF1 regulator of nonsense transcripts homolog UPF1 RNA helicase and ATPase Yeast Upf1p homolog
Images
ET7108-70_1.jpg Fig1: Western blot analysis of hUPF1 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET7108-70_2.jpg Fig2: ICC staining of hUPF1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-70, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-70_3.jpg Fig3: ICC staining of hUPF1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-70, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-70_4.jpg Fig4: ICC staining of hUPF1 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-70, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-70_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-hUPF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-70_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-hUPF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-70_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-hUPF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-70_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-hUPF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-70, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-70_9.jpg Fig9: Flow cytometric analysis of hUPF1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-70, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.