Anti-hUPF1 antibody [JG38-44]
cat.: ET7108-70
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IF, IP, IHC-P, FC
Clonality: Monoclonal
Clone number: JG38-44
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 124 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal Human hUPF1 .
Positive control: PC-3M, A431, LOVO, human colon tissue, human prostate cancer tissue, human kidney tissue, mouse brain tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC
  IP

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
1:10-1:50
Uniprot #: SwissProt: Q92900 Human | Q9EPU0 Mouse
Alternative names: ATP dependent helicase RENT1 ATP-dependent helicase RENT1 Delta helicase FLJ43809 FLJ46894 HUPF 1 hUpf1 KIAA0221 Nonsense mRNA reducing factor 1 NORF 1 NORF1 pNORF 1 pNORF1 Regulator of nonsense transcripts 1 RENT 1 RENT1 RENT1_HUMAN Smg 2 Smg 2 homolog nonsense mediated mRNA decay factor UP Frameshift 1 Up frameshift mutation 1 homolog (S. cerevisiae) Up frameshift mutation 1 homolog Up frameshift suppressor 1 homolog Up-frameshift suppressor 1 homolog UPF 1 UPF 1 regulator of nonsense transcripts homolog upf1 UPF1 regulator of nonsense transcripts homolog UPF1 RNA helicase and ATPase Yeast Upf1p homolog
Images
ET7108-70_1.jpg Fig1: Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1,000 dilution.
ET7108-70_2.jpg Fig2: ICC staining hUPF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7108-70_3.jpg Fig3: ICC staining hUPF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7108-70_4.jpg Fig4: ICC staining hUPF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7108-70_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.
ET7108-70_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-hUPF1 antibody. Counter stained with hematoxylin.
ET7108-70_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.
ET7108-70_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.
ET7108-70_9.jpg Fig9: Flow cytometric analysis of PC-3M cells with hUPF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.