Cdx1 Recombinant Rabbit Monoclonal Antibody [JG38-50]
cat.: ET7108-71
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG38-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cdx1 aa 1-49 / 265. |
| Positive control: | Human small intestine tissue lysate, CRC cell lysate, LOVO, human colon carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:200 1:200 |
| Uniprot #: | SwissProt: P47902 Human |
| Alternative names: | Caudal type homeo box transcription factor 1 Caudal type homeobox 1 Caudal type homeobox protein Caudal type homeobox protein CDX1 Caudal type homeobox transcription factor 1 Caudal-type homeobox protein 1 CDX1 CDX1_HUMAN Homeobox protein CDX 1 Homeobox protein CDX-1 MGC116915 OTTHUMP00000160524 |
Images
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Fig1:
Western blot analysis of Cdx1 on different lysates with Rabbit anti-Cdx1 antibody (ET7108-71) at 1/1,000 dilution. Lane 1: human small intestine tissue lysates (20 µg/Lane) Lane 2: CRC cell lysates (10 µg/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-71) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of LOVO cells labeling Cdx1 with Rabbit anti-Cdx1 antibody (ET7108-71) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cdx1 antibody (ET7108-71) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cdx1 antibody (ET7108-71) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-71) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.