NOXA2 Recombinant Rabbit Monoclonal Antibody [JG86-31]
cat.: ET7108-72
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JG86-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NOXA2 aa 180-320 / 526. |
| Positive control: | Rat spleen tissue lysates, THP-1 cell lysate, rat spleen tissue, human spleen tissue, mouse spleen tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: P19878 Human | O70145 Mouse | A7E3N2 Rat |
| Alternative names: | 67 kDa neutrophil oxidase factor Chronic granulomatous disease autosomal 2 FLJ93058 NADPH oxidase activator 2 NCF-2 Ncf2 NCF2_HUMAN Neutrophil cytosol factor 2 Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) Neutrophil NADPH oxidase factor 2 NOXA2 P67 PHOX p67-phox p67phox |
Images
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Fig1:
Western blot analysis of NOXA2 on different lysates with Rabbit anti-NOXA2 antibody (ET7108-72) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: rat spleen tissue lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7108-72, 1/5,000 in primary antibody dilution buffer (K1803), room temperature for 2 hours Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 60 kDa Observed band size: 67 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.