Nudel Recombinant Rabbit Monoclonal Antibody [JG38-63]
cat.: ET7108-73
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG38-63 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Nudel aa 1-49 / 345. |
| Positive control: | Rat brain tissue lysates, human liver cancer tissue, human small intestine tissue, human uterus tissue. |
| Subcellular location: | Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9GZM8 Human | Q9ERR1 Mouse | Q78PB6 Rat |
| Alternative names: | A. nidulans DKFZp451M0318 ENDOOLIGOPEPTIDASE A EOPA MITAP 1 MITAP1 Mitosin associated protein 1 Mitosin associated protein MITAP1 Mitosin-associated protein 1 Ndel 1 NDEL1 NDEL1_HUMAN Nuclear distribution gene E like homolog 1 Nuclear distribution protein nudE like 1 Nuclear distribution protein nudE-like 1 NUDE like protein NudE nuclear distribution gene E homolog like 1 A. nidulans NudE nuclear distribution gene E homolog like 1 NUDEL Protein Nudel |
Images
|
Fig1:
Western blot analysis of Nudel on rat brain tissue lysates with Rabbit anti-Nudel antibody (ET7108-73) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-73) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-Nudel antibody (ET7108-73) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-73) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Nudel antibody (ET7108-73) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-73) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human uterus tissue with Rabbit anti-Nudel antibody (ET7108-73) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-73) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.