Tropomyosin 1 Recombinant Rabbit Monoclonal Antibody [JG38-66]
cat.: ET7108-74
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JG38-66
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 33 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Tropomyosin 1 aa 168-284 / 284.
Positive control: Rat heart tissue lysate, mouse heart tissue lysate, rat smooth muscle tissue, human heart tissue, mouse skeletal muscle tissue, Hela.
Subcellular location: Cytoskeleton.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P09493 Human | P58771 Mouse | P04692 Rat
Alternative names: AA986836 AI854628 Alpha tropomyosin alpha-TM Alpha-tropomyosin C15orf13 cardiomyopathy, hypertrophic 3 CMD1Y CMH3 HTM alpha HTM-alpha OTTHUMP00000163688 sarcomeric tropomyosin kappa TM2 Tmpa TMSA Tpm-1 TPM1 TPM1_HUMAN tropomyosin 1 (alpha) tropomyosin 1 (alpha) isoform 1 tropomyosin 1 (alpha) isoform 2 tropomyosin 1 (alpha) isoform 3 tropomyosin 1 (alpha) isoform 4 tropomyosin 1 (alpha) isoform 5 tropomyosin 1 (alpha) isoform 6 tropomyosin 1 (alpha) isoform 7 Tropomyosin 1 Tropomyosin alpha 1 chain Tropomyosin alpha-1 chain Tropomyosin, skeletal muscle alpha Tropomyosin-1
Images
ET7108-74_1.jpg Fig1: Western blot analysis of Tropomyosin 1 on different lysates with Rabbit anti-Tropomyosin 1 antibody (ET7108-74) at 1/1,000 dilution.

Lane 1: Rat heart tissue lysate
Lane 2: Mouse heart tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 33 kDa
Observed band size: 35 kDa

Exposure time: 46 seconds;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-74) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET7108-74_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-74_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-74_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-74_5.jpg Fig5: Flow cytometric analysis of Tropomyosin 1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-74, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.