Ube2N Recombinant Rabbit Monoclonal Antibody [JG38-68]
cat.: ET7108-75
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JG38-68
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 17 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Ube2N aa 1-93 / 152.
Positive control: Daudi, SH-SY-5Y, mouse spleen tissue lysate, rat spleen tissue lysate, human colon tissue, human prostate cancer tissue, mouse testis tissue.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB

1:1,000-1:2,000
Uniprot #: SwissProt: P61088 Human | P61089 Mouse | Q9EQX9 Rat
Alternative names: Bendless like ubiquitin conjugating enzyme Bendless-like ubiquitin-conjugating enzyme BLU EC 6.3.2.19 Epididymis secretory protein Li 71 HEL-S-71 Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds MGC131857 MGC8489 UBC 13 Ubc13 UbCH ben UbcH-ben UbcH13 UBCHBEN Ube 2N Ube2n UBE2N_HUMAN Ubiquitin carrier protein N Ubiquitin conjugating enzyme E2 N Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) Ubiquitin conjugating enzyme E2N Ubiquitin protein ligase N Ubiquitin-conjugating enzyme E2 N Ubiquitin-protein ligase N Yeast UBC13 homolog
Images
ET7108-75_1.jpg Fig1: Western blot analysis of Ube2N on different lysates with Rabbit anti-Ube2N antibody (ET7108-75) at 1/1,000 dilution.

Lane 1: Daudi (Human Burkitt's lymphoma cell)
Lane 2: Rat spleen

Lysates/proteins at 15 µg/Lane.
Exposure time: 20 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7108-75, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 17 kDa
Observed band size: 17 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.