Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JG38-70 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 12 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human DAP12 aa 1-113 / 113. |
Positive control: | THP-1 cell lysates, human lung carcinoma tissue, K562. |
Subcellular location: | Membrane. |
Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: O43914 Human |
Alternative names: | DAP 12 DAP12 DNAX activation protein 12 DNAX-activation protein 12 KAR-associated protein KARAP Killer activating receptor associated protein Killer-activating receptor-associated protein PLOSL TYOBP_HUMAN TYRO protein tyrosine kinase binding protein TYRO protein tyrosine kinase-binding protein TYROBP |
Fig1: Western blot analysis of DAP12 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. | |
Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-DAP12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-76, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. | |
Fig3: Flow cytometric analysis of DAP12 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-76, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow). |