Carbonic Anhydrase I Recombinant Rabbit Monoclonal Antibody [JG38-71]
cat.: ET7108-77
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG38-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Carbonic Anhydrase I aa 120-261 / 261. |
| Positive control: | TF-1 cell lysate, K-562 cell lysate, zebrafish tissue lysate, human lung tissue lysate, mouse colon tissue lysate, rat bone marrow tissue lysate, human spleen tissue, human small intestine tissue, mouse spleen tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:800 |
| Uniprot #: | SwissProt: P00915 Human | P13634 Mouse | B0BNN3 Rat |
| Alternative names: | CA 1 CA I CA-I CA1 CAB CAH1_HUMAN CAI CAN Car 1 Car1 Carbonate dehydratase I Carbonic anhydrase 1 Carbonic anhydrase A Carbonic anhydrase B Carbonic anhydrase B, formerly Carbonic anhydrase I Carbonic dehydratase ECK0125 JW0122 yadF |
Images
|
Fig1:
Western blot analysis of Carbonic Anhydrase I on different lysates with Rabbit anti-Carbonic Anhydrase I antibody (ET7108-77) at 1/1,000 dilution. Lane 1: TF-1 cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: Zebrafish tissue lysate (40 µg/Lane) Lane 4: Human lung tissue lysate (40 µg/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-77) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Carbonic Anhydrase I on different lysates with Rabbit anti-Carbonic Anhydrase I antibody (ET7108-77) at 1/500 dilution. Lane 1: Mouse colon tissue lysate Lane 2: Rat bone marrow tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-77) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Carbonic Anhydrase I antibody (ET7108-77) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-77) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Carbonic Anhydrase I antibody. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Carbonic Anhydrase I antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.