TNPO3 Recombinant Rabbit Monoclonal Antibody [JG38-77]
cat.: ET7108-80
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IF-Tissue
Clonality: Monoclonal
Clone number: JG38-77
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 104 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human TNPO3 aa 830-923 / 923.
Positive control: K-562 cell lysate, HL-60 cell lysate, HeLa cell lysate, Ramos cell lysate, NIH/3T3 cell lysate, Mouse pancreas tissue lysate, Rat pancreas tissue lysate, mouse kidney tissue, rat kidney tissue, human small intestine tissue, K-562.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IF-Tissue
1:2,000
1:50-1:200
1:1,000
1:50
Uniprot #: SwissProt: Q9Y5L0 Human | Q6P2B1 Mouse
Entrez Gene: 296954 Rat
Alternative names: TNPO 3 Imp12 Importin 12 Importin-12 IPO12 MTR10A TNPO3 TNPO3_HUMAN Transportin 3 Transportin SR Transportin-3 Transportin-SR TRN SR TRN-SR TRN-SR2 TRNSR
Images
ET7108-80_1.jpg Fig1: Western blot analysis of TNPO3 on different lysates with Rabbit anti-TNPO3 antibody (ET7108-80) at 1/2,000 dilution.

Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: HL-60 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: Ramos cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 7: Rat pancreas tissue lysate (40 µg/Lane)

Predicted band size: 104 kDa
Observed band size: 100 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-80) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7108-80_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-TNPO3 antibody (ET7108-80) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-80) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-80_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TNPO3 antibody (ET7108-80) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-80) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-80_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TNPO3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-80, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-80_5.jpg Fig5: Flow cytometric analysis of K-562 cells labeling TNPO3.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7108-80, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.