p54nrb/NONO Recombinant Rabbit Monoclonal Antibody [JG87-38]
cat.: ET7108-81
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JG87-38
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human p54nrb aa 365-471 / 471.
Positive control: 293, SH-SY-5Y, SiHa, rat kidney tissue, human liver tissue, human colon tissue, human prostate cancer tissue, mouse testis tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:500-1:2,000
1:50
1:50-1:200
Uniprot #: SwissProt: Q15233 Human | Q99K48 Mouse | Q5FVM4 Rat
Alternative names: 52 kDa subunit 54 kDa nuclear RNA and DNA binding protein 54 kDa nuclear RNA- and DNA-binding protein 55 kDa nuclear protein DNA binding p52/p100 complex 52 kDa subunit DNA-binding p52/p100 complex NMT 55 NMT55 Non Pou domain containing octamer (ATGCAAAT) binding protein Non POU domain containing octamer binding Non POU domain containing octamer binding protein Non-POU domain-containing octamer-binding protein Nono NonO protein NONO_HUMAN NRB 54 NRB NRB54 Nuclear RNA binding protein 54kD P54 p54(nrb) p54nrb PPP1R114 Protein phosphatase 1 regulatory subunit 114
Images
ET7108-81_1.jpg Fig1: Western blot analysis of p54nrb on 293 (1) and SH-SY-5Y (2) cell lysate using anti-p54nrb antibody at 1/500 dilution.
ET7108-81_2.jpg Fig2: ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7108-81_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti- p54nrb antibody. Counter stained with hematoxylin.
ET7108-81_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- p54nrb antibody. Counter stained with hematoxylin.
ET7108-81_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- p54nrb antibody. Counter stained with hematoxylin.
ET7108-81_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-p54nrb antibody. Counter stained with hematoxylin.
ET7108-81_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- p54nrb antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.