p54nrb/NONO Recombinant Rabbit Monoclonal Antibody [JG87-38]
cat.: ET7108-81
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG87-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human p54nrb aa 365-471 / 471. |
| Positive control: | 293, SH-SY-5Y, SiHa, rat kidney tissue, human liver tissue, human colon tissue, human prostate cancer tissue, mouse testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50 1:50-1:200 |
| Uniprot #: | SwissProt: Q15233 Human | Q99K48 Mouse | Q5FVM4 Rat |
| Alternative names: | 52 kDa subunit 54 kDa nuclear RNA and DNA binding protein 54 kDa nuclear RNA- and DNA-binding protein 55 kDa nuclear protein DNA binding p52/p100 complex 52 kDa subunit DNA-binding p52/p100 complex NMT 55 NMT55 Non Pou domain containing octamer (ATGCAAAT) binding protein Non POU domain containing octamer binding Non POU domain containing octamer binding protein Non-POU domain-containing octamer-binding protein Nono NonO protein NONO_HUMAN NRB 54 NRB NRB54 Nuclear RNA binding protein 54kD P54 p54(nrb) p54nrb PPP1R114 Protein phosphatase 1 regulatory subunit 114 |
Images
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Fig1:
Western blot analysis of p54nrb/NONO on different lysates with Rabbit anti-p54nrb/NONO antibody (ET7108-81) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si p54nrb/NONO cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 60 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-81) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of p54nrb on 293 (1) and SH-SY-5Y (2) cell lysate using anti-p54nrb antibody at 1/500 dilution. |
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Fig3: ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti- p54nrb antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- p54nrb antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- p54nrb antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-p54nrb antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- p54nrb antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.