Bub3 Recombinant Rabbit Monoclonal Antibody [JG38-81]
cat.: ET7108-82
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IP, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG38-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Bub3 aa 2-50 / 328. |
| Positive control: | A549, HL-60, A431, rat colon tissue lysate, LOVO, rat testis tissue, human thyroid gland tissue, human colon cancer tissue, mouse fallopian tissue, PC-3M. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P IP FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:10-1:50 1:50-1:100 |
| Uniprot #: | SwissProt: O43684 Human | Q9WVA3 Mouse |
| Alternative names: | Aa2-050 AU019800 AU021329 AU043350 AW146323 BUB 3 BUB 3L BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog bub3 BUB3 budding uninhibited by benzimidazoles 3 homolog BUB3 mitotic checkpoint protein BUB3_HUMAN BUB3L Budding uninhibited by benomyl Budding uninhibited by benzimidazoles 3 Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) Budding uninhibited by benzimidazoles 3 homolog (yeast) Budding uninhibited by benzimidazoles 3 homolog Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of C78067 hBUB3 Mitotic checkpoint component Mitotic checkpoint protein bub3 OTTHUMP00000020679 OTTHUMP00000020680 WD repeat type I transmembrane protein A72.5 |
Images
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Fig1:
Western blot analysis of Bub3 on different lysates using anti-Bub3 antibody at 1/500 dilution. Positive control: Lane 1: A549 Lane 2: HL-60 Lane 3: A431 Lane 4: Rat colon |
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Fig2: ICC staining Bub3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Bub3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Bub3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Bub3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Bub3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bub3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse fallopian tissue using anti-Bub3 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of PC-3M cells with Bub3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.