p150 CAF1 Recombinant Rabbit Monoclonal Antibody [JG38-98]
cat.: ET7108-83
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IP, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG38-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 107 kDa (Predicted band size) |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human p150 CAF1 aa 81-195 / 956. |
| Positive control: | K562 cell lysates, A431 cell lysates, 293T, A431, LOVO, human tonsil tissue, human colon tissue, Daudi, human testis tissue, human lymph nodes tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:10-1:50 1:50-1:100 |
| Uniprot #: | SwissProt: Q13111 Human |
| Alternative names: | CAF 1 CAF 1 subunit A CAF CAF I 150 kDa subunit CAF I p150 CAF-1 subunit A CAF-I 150 kDa subunit CAF-I p150 CAF1 CAF1A_HUMAN CAF1B CAF1P150 CHAF1A Chromatin assembly factor 1 subunit A Chromatin Assembly Factor 1 Subunit A p150 Chromatin assembly factor I (150 kDa) Chromatin assembly factor I 150 kDa Chromatin assembly factor I p150 subunit DCAF1 hp150 MGC71229 P150 |
Images
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Fig1: Western blot analysis of p150 CAF1 on K562 (1) and A431 (2) cell lysate using anti-p150 CAF1 antibody at 1/1,000 dilution. |
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Fig2: ICC staining p150 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining p150 CAF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining p150 CAF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p150 CAF1 antibody. Counter stained with hematoxylin. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-p150 CAF1 antibody (ET7108-83) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-83) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Flow cytometric analysis of Daudi cells with p150 CAF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-p150 CAF1 antibody (ET7108-83) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-83) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-p150 CAF1 antibody (ET7108-83) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-83) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.