ORP150 Recombinant Rabbit Monoclonal Antibody [JG39-11]
cat.: ET7108-84
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG39-11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 111 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ORP150 aa 1-190 / 999. |
| Positive control: | SH-SY5Y cell lysates, mouse prostate tissue lysates, rat testis tissue, human liver cancer tissue, human small intestine tissue, mouse testis tissue. |
| Subcellular location: | Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P FC |
1:5,000-1:20,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y4L1 Human | Q9JKR6 Mouse |
| Alternative names: | 150 kDa oxygen regulated protein 150 kDa oxygen-regulated protein 170 kDa glucose regulated protein 170 kDa glucose-regulated protein DKFZp686N08236 FLJ94899 FLJ97572 glucose regulated protein 170 Grp 170 GRP-170 Grp170 HSP 12A HSP12A Hyou1 HYOU1_HUMAN Hypoxia up regulated 1 hypoxia up-regulated 1 precursor Hypoxia up-regulated protein 1 Hypoxia upregulated 1 Orp 150 ORP-150 Orp150 oxygen regulated protein (150kD) |
Images
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Fig1:
Western blot analysis of ORP150 on SH-SY5Y cell lysates with Rabbit anti-ORP150 antibody (ET7108-84) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 111 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-84) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ORP150 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ORP150 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of HepG2 cells with ORP150 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.