p60 CAF1 Recombinant Rabbit Monoclonal Antibody [JG92-30]
cat.: ET7108-85
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IP, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG92-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human p60 CAF1 aa 419-559 / 559. |
| Positive control: | SiHa, K562, A431, 293T, human tonsil tissue, human appendix tissue, human colon tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:10-1:50 1:50-1:100 |
| Uniprot #: | SwissProt: Q13112 Human |
| Alternative names: | CAF 1 subunit B CAF 1A CAF I 60 kDa subunit CAF IP60 CAF-1 CAF-1 subunit B CAF-I 60 kDa subunit CAF-I p60 CAF1 CAF1A CAF1B_HUMAN CAF1P60 CHAF 1B CHAF1B Chromatin assembly factor 1 subunit B Chromatin assembly factor I Chromatin assembly factor I p60 subunit Human chromatin assembly factor-I p60 subunit M phase phosphoprotein 7 M-phase phosphoprotein 7 MPHOSPH7 MPP 7 MPP7 p60 subunit |
Images
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Fig1:
Western blot analysis of p60 CAF1 on different cell lysates using anti-p60 CAF1 antibody at 1/1,000 dilution. Positive control: Lane 1: SiHa Lane 2: K562 Lane 3: A431 |
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Fig2: ICC staining p60 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining p60 CAF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining p60 CAF1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p60 CAF1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p60 CAF1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-p60 CAF1 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of K562 cells with p60 CAF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.