p60 CAF1 Recombinant Rabbit Monoclonal Antibody [JG92-30]
cat.: ET7108-85
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG92-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human p60 CAF1 aa 419-559 / 559. |
| Positive control: | SiHa cell lysates, K562 cell lysates, A431cell lysates, human tonsil tissue, human colon tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:500-1:2,000 1:50-1:200 1:1,00 1:10-1:50 |
| Uniprot #: | SwissProt: Q13112 Human |
| Alternative names: | CAF 1 subunit B CAF 1A CAF I 60 kDa subunit CAF IP60 CAF-1 CAF-1 subunit B CAF-I 60 kDa subunit CAF-I p60 CAF1 CAF1A CAF1B_HUMAN CAF1P60 CHAF 1B CHAF1B Chromatin assembly factor 1 subunit B Chromatin assembly factor I Chromatin assembly factor I p60 subunit Human chromatin assembly factor-I p60 subunit M phase phosphoprotein 7 M-phase phosphoprotein 7 MPHOSPH7 MPP 7 MPP7 p60 subunit |
Images
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Fig1:
Western blot analysis of p60 CAF1 on different cell lysates using anti-p60 CAF1 antibody at 1/1,000 dilution. Positive control: Lane 1: SiHa Lane 2: K562 Lane 3: A431 |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-p60 CAF1 antibody (ET7108-85) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-85) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-p60 CAF1 antibody (ET7108-85) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-85) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.