ADAMTS13 Recombinant Rabbit Monoclonal Antibody [JG39-21]
cat.: ET7108-86
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG39-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size 154 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ADAMTS13 aa 71-124 / 1,427. |
| Positive control: | SiHa cell lysate, PC-3M cell lysate, A549 cell lysate, human kidney tissue, rat kidney tissue, human liver tissue, human colon tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q76LX8 Human Entrez Gene: 102554393 Rat |
| Alternative names: | A disintegrin and metalloproteinase with thrombospondin motifs 13 A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 A disintegrin like and metalloprotease with thrombospondin type 1 motif 13 ADAM metallopeptidase with thrombospondin type 1 motif 13 ADAM TS ADAM-TS 13 ADAM-TS13 ADAMTS 13 ADAMTS-13 ADAMTS13 ADAMTS13 protein ATS13_HUMAN C9orf8 TTP Von Willebrand factor cleaving protease von Willebrand factor-cleaving protease vWF cleaving protease vWF CP vWF-cleaving protease vWF-CP vWFCP |
Images
|
Fig1:
Western blot analysis of ADAMTS13 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-86, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate Lane 2: PC-3M cell lysate Lane 3: A549 cell lysate |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ADAMTS13 antibody (ET7108-86) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-86) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ADAMTS13 antibody (ET7108-86) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-86) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ADAMTS13 antibody (ET7108-86) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-86) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ADAMTS13 antibody (ET7108-86) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-86) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.