CBFb Recombinant Rabbit Monoclonal Antibody [JG39-35]
cat.: ET7108-87
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG39-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CBFb aa 60-182 / 182. |
| Positive control: | Rat kidney tissue, human kidney tissue, SiHa, mouse lung tissue, mouse hippocampus tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:50-1:400 1:50-1:100 |
| Uniprot #: | SwissProt: Q13951 Human | Q08024 Mouse Entrez Gene: 361391 Rat |
| Alternative names: | CBF b CBF beta CBF-beta CBFB CBFbeta Core binding factor beta subunit core binding factor subunit beta Core-binding factor subunit beta PEA 2 PEA2 PEA2 beta PEA2-beta PEA2beta PEBB_HUMAN PEBP 2B PEBP2 beta PEBP2-beta PEBP2B PEBP2beta Polyomavirus enhancer binding protein 2 beta subunit Polyomavirus enhancer-binding protein 2 beta subunit SL3 3 enhancer factor 1 beta subunit SL3 3 enhancer factor 1 subunit beta SL3-3 enhancer factor 1 subunit beta SL3/AKV core binding factor beta subunit SL3/AKV core-binding factor beta subunit |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CBFb antibody (ET7108-87) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-87) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin. |
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Fig3: Flow cytometric analysis of SiHa cells with CBFb antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-CBFb antibody (ET7108-87) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-87) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CBFb antibody (ET7108-87) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-87) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.