COMT Recombinant Rabbit Monoclonal Antibody [JG39-48]
cat.: ET7108-89
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG39-48 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30/24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within COMT aa 11-107 / 271. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, HepG2 cell lysate, rat testis tissue lysate, mouse testis tissue. |
| Subcellular location: | Cell membrane. Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: P21964 Human | O88587 Mouse | P22734 Rat |
| Alternative names: | Catechol O methyltransferase Catechol O-methyltransferase COMT COMT_HUMAN EC 2.1.1.6 |
Images
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Fig1:
Western blot analysis of COMT on different lysates with Rabbit anti-COMT antibody (ET7108-89) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30/24 kDa Observed band size: 30/24 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-89) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-COMT antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.