SUN2 Recombinant Rabbit Monoclonal Antibody [JG39-52]
cat.: ET7108-92
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JG39-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SUN2 aa 591-717 / 717. |
| Positive control: | Saos-2 cell lysate, HeLa cell lysate, HepG2 cell lysate, A431 cell lysate, A431, human testis tissue, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Endosome. Nucleus inner membrane. |
| Recommended Dilutions:
WB IHC-P FC IF-Cell IF-Tissue |
1:2,000 1:1,000 1:1,000 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: Q9UH99 Human | Q8BJS4 Mouse |
| Alternative names: | FRIGG KIAA0668 nuclear envelope protein Protein unc-84 homolog B Rab5 interacting protein RAB5IP Sad1 and UNC84 domain containing 2 Sad1 unc-84 domain protein 2 Sad1 unc84 domain protein Sad1/unc-84 protein-like 2 Sad1/unc84 protein-like SUN domain-containing protein 2 UNC 84B unc84 homolog B (C. elegans) unc84 homolog B unc84, C. elegans, homolog of, B UNC84B |
Images
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Fig1:
Western blot analysis of SUN2 on different lysates with Rabbit anti-SUN2 antibody (ET7108-92) at 1/2,000 dilution. Lane 1: Saos-2 cell lysate Lane 2: HeLa cell lysate Lane 3: HepG2 cell lysate Lane 4: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 80 kDa Observed band size: 75 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-92) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A431 cells labeling SUN2 with Rabbit anti-SUN2 antibody (ET7108-92) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SUN2 antibody (ET7108-92) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SUN2 antibody (ET7108-92) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-92) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SUN2 antibody (ET7108-92) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-92) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SUN2 antibody (ET7108-92) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-92) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Flow cytometric analysis of A431 cells labeling SUN2. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7108-92, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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