SUN2 Recombinant Rabbit Monoclonal Antibody [JG39-52]
cat.: ET7108-92
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JG39-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 80 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SUN2 aa 591-717 / 717. |
| Positive control: | A431 cell lysate, rat epididymis tissue, human tonsil tissue, human colon tissue, human prostate cancer tissue, mouse liver tissue, SK-Br-3. |
| Subcellular location: | Endosome. Nucleus inner membrane. |
| Recommended Dilutions:
WB IHC-P FC IF-Cell IF-Tissue |
1:500-1:2,000 1:50-1:200 1:50-1:100 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UH99 Human | Q8BJS4 Mouse |
| Alternative names: | FRIGG KIAA0668 nuclear envelope protein Protein unc-84 homolog B Rab5 interacting protein RAB5IP Sad1 and UNC84 domain containing 2 Sad1 unc-84 domain protein 2 Sad1 unc84 domain protein Sad1/unc-84 protein-like 2 Sad1/unc84 protein-like SUN domain-containing protein 2 UNC 84B unc84 homolog B (C. elegans) unc84 homolog B unc84, C. elegans, homolog of, B UNC84B |
Images
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Fig1:
Western blot analysis of SUN2 on A431 cell lysates with Rabbit anti-SUN2 antibody (ET7108-92) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 80 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-92) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SUN2 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SUN2 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUN2 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SUN2 antibody. Counter stained with hematoxylin. |
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Fig6: ICC staining SUN2 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SUN2 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of SK-Br-3 cells with SUN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.