FKBP52 Recombinant Rabbit Monoclonal Antibody [JG96-30]
cat.: ET7108-93
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG96-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FKBP52 aa 1-132 / 459. |
| Positive control: | SK-Br-3 cell lysate, 293 cell lysate, 293T, MCF-7, human prostate cancer tissue, human esophagus tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm. Cytoskeleton. Microtubule. Mitochondrion. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q02790 Human | P30416 Mouse |
| Alternative names: | 51 kDa FK506-binding protein 52 kDa FK506 binding protein 52 kDa FK506-binding protein 52 kDa FKBP 59 kDa immunophilin FK506 binding protein 4 FK506-binding protein 4 FKBP 4 FKBP 52 FKBP 59 FKBP-4 FKBP-52 FKBP4 FKBP4_HUMAN FKBP51 FKBP52 protein FKBP59 HBI Hsp 56 HSP binding immunophilin HSP-binding immunophilin Hsp56 Immunophilin FKBP52 N-terminally processed p52 p59 p59 protein Peptidyl prolyl cis trans isomerase Peptidyl-prolyl cis-trans isomerase FKBP4 Peptidylprolyl cis trans isomerase PPIase PPIase FKBP4 Rotamase T cell FK506 binding protein 59kD |
Images
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Fig1:
Western blot analysis of FKBP52 on different lysates with Rabbit anti-FKBP52 antibody (ET7108-93) at 1/500 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: 293 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-93) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining FKBP52 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining FKBP52 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-FKBP52 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-FKBP52 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FKBP52 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of 293T cells with FKBP52 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.