FKBP52 Recombinant Rabbit Monoclonal Antibody [JG96-30]
cat.: ET7108-93
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JG96-30
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 52 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human FKBP52 aa 1-132 / 459.
Positive control: SK-Br-3 cell lysate, HeLa cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human prostate cancer tissue, 293T, MCF-7.
Subcellular location: Cytoplasm. Cytoskeleton. Microtubule. Mitochondrion. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:2,000
1:50-1:200
1:1,000
1:1,000
Uniprot #: SwissProt: Q02790 Human | P30416 Mouse | Q9QVC8 Rat
Alternative names: 51 kDa FK506-binding protein 52 kDa FK506 binding protein 52 kDa FK506-binding protein 52 kDa FKBP 59 kDa immunophilin FK506 binding protein 4 FK506-binding protein 4 FKBP 4 FKBP 52 FKBP 59 FKBP-4 FKBP-52 FKBP4 FKBP4_HUMAN FKBP51 FKBP52 protein FKBP59 HBI Hsp 56 HSP binding immunophilin HSP-binding immunophilin Hsp56 Immunophilin FKBP52 N-terminally processed p52 p59 p59 protein Peptidyl prolyl cis trans isomerase Peptidyl-prolyl cis-trans isomerase FKBP4 Peptidylprolyl cis trans isomerase PPIase PPIase FKBP4 Rotamase T cell FK506 binding protein 59kD
Images
ET7108-93_1.jpg Fig1: Western blot analysis of FKBP52 on different lysates with Rabbit anti-FKBP52 antibody (ET7108-93) at 1/2,000 dilution.

Lane 1: SK-Br-3 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Mouse testis tissue lysate (40 µg/Lane)
Lane 3: Rat testis tissue lysate (40 µg/Lane)

Predicted band size: 52 kDa
Observed band size: 55 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-93) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7108-93_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-FKBP52 antibody (ET7108-93) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-93) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-93_3.jpg Fig3: ICC staining FKBP52 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7108-93_4.jpg Fig4: ICC staining FKBP52 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7108-93_5.jpg Fig5: Flow cytometric analysis of 293T cells labeling FKBP52.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7108-93, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.