Methionine Aminopeptidase 2 Recombinant Rabbit Monoclonal Antibody [JG39-79]
cat.: ET7108-97
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JG39-79
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size 53 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Methionine Aminopeptidase 2 aa 340-478 / 478.
Positive control: Daudi cell lysate, K562 cell lysate, mouse thymus tissue lysate, mouse kidney tissue lysate, LOVO, PC-3M, SH-SY5Y, rat seminal vesicle tissue, human tonsil tissue, human prostate carcinoma tissue, human kidney tissue, mouse testis tissue, Daudi.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P50579 Human | O08663 Mouse | P38062 Rat
Alternative names: A930035J23Rik AI047573 AL024412 Amp2 AU014659, EIF 2 associated p67 homolog EIF-2-associated p67 Initiation factor 2 associated 67 kDa glycoprotein Initiation factor 2-associated 67 kDa glycoprotein MAP 2 MAP2 MAP2_HUMAN MetAP 2 Metap2 Methionine aminopeptidase 2 Methionyl aminopeptidase 2 MGC102452 MGC127390 MGC53792 MNPEP p67 p67eIF2 Peptidase M Peptidase M2
Images
ET7108-97_1.jpg Fig1: Western blot analysis of Methionine Aminopeptidase 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-97, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Daudi cell lysate
Lane 2: K562 cell lysate
Lane 3: Mouse thymus tissue lysate
Lane 4: Mouse kidney tissue lysate
ET7108-97_2.jpg Fig2: ICC staining of Methionine Aminopeptidase 2 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-97_3.jpg Fig3: ICC staining of Methionine Aminopeptidase 2 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-97_4.jpg Fig4: ICC staining of Methionine Aminopeptidase 2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-97, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7108-97_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat seminal vesicle tissue using anti-Methionine Aminopeptidase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-97_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Methionine Aminopeptidase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-97_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-Methionine Aminopeptidase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-97_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Methionine Aminopeptidase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-97_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Methionine Aminopeptidase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-97, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7108-97_10.jpg Fig10: Flow cytometric analysis of Methionine Aminopeptidase 2 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-97, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.